The Whole Picture: Total RNA Sequencing

Total RNA sequencing        It is well known that scientists have been interested in studying the transcriptome of organisms since the popularization of microarrays. However, a major drawback of transcriptome analysis utilizing microarray technology is the requirement that the target of interest must already be mapped. The introduction of Next Generation Sequencing (NGS) has been able to provide researchers a new tool to study the transcriptome, one that is able to increase sensitivity as well as identify those rare variants that are unknown to the researchers. Laboratories around the world, including MR DNA, are able to utilize NGS instruments such as Illumina’s MiSeq and HiSeq 2500 to aid researchers in grasping a greater picture of the whole transcriptome using Total RNA sequencing technology. By sequencing the whole transcriptome, the highly experienced team at MR DNA is able detect differences in gene expression between samples as well as provide de novo RNA-seq assembly. For your next transcriptome study, contact the scientists at MR DNA , and see how you can to take advantage of their affordable cost and rapid turnaround time.

 

Next Generation Sequencing | MR DNA

MR DNA can perform whole-genome or de novo sequencing, resequencing … in one flow cell lane, reducing the cost of this service for small genomic libraries. …transcriptome analysis using mRNA sequencing on the HiSeq platform.

Price List | MR DNA

TruSeq RNA/small RNA sample library prep, per sample … Illumina HiSeq 2000/2500sequencing (high-output mode) 7 lanes run with samples, 1 phiX lane

 

Next Generation Sequencing Facility – MR DNA

The DNA Sequencing Facility offers all-inclusive next generation sequencing, DNA extraction and SNP genotyping services on a … We work with our customers to prepare DNA, RNA, ChIP, GBS, metagenomic, exome, and other sequencing … Run Charges – HiSeq 2000/2500 High Throughput  …

RNA-Seq (Quantification) | MR DNA

Powered by our unique bioinformatics capabilities, MR DNA RNASeq services deliver …RNASeq based on Hiseq: high-throughput and a wide  …

 

Sequencing – Genome Technology | MR DNA

https://mrdnalab.com

… instructions. Below you will find more information about our sequencing services: library preparation, sequencing, and analyses. … mRNASeq · ChIP-Seq …Sequencing. GTAC operates four Illumina HiSeq 2500 sequencing instruments.

 

MR DNA: HiSeq 2500 & MiSeq – Next Generation Sequencing

The MR DNA Facility houses an Illumina HiSeq 2500 and an … such as ChIP-Seq, transcript counts, SNP detection, and small RNA analysis, the … the mate-pair protocol and should contact the facility regarding this service.

 

Epigenetics via ChIP-Seq (Chromatin Immunoprecipitation Sequencing)

As technology progresses, so does the curiosity of scientific minds around the globe. As more and more scientists shift from traditional DNA sequencing methods to massively parallel DNA sequencing, the new discoveries are increasing at an exponential rate. Such discoveries come with the popularization of chromatin immunoprecipitation sequencing or ChIP-Seq. ChIP sequencing is able to take scientists who were once excited to decipher the genomic code and stimulate new discoveries by determining how those genes are expressed. Laboratories, such as MR DNA, are assisting researchers from universities around the world to pinpoint how DNA and/or RNA are interacting with certain proteins by employing the ChIP-Seq protocol on a routine basis. Contact the highly experienced team at MR DNA in order to take advantage of their affordable cost and rapid turnaround time for your next epigenetic study.

 

Our services – MR DNA Laboratory

MR DNA

Illumina HiSeq 2500/200, MiSeq – The HiSeq 2500/2000 sequencing systems offer the … Metagenomics and amplicon sequencing; ChIPSeq  …

 

Functional Genomics

MR DNA

MR DNA offers library prep, sequencing, and basic data analysis services for …Simple ChIPSeq … hiSeq2500, …., sequence the samples

 

High-Throughput Sequencing Center

MR DNA

We provide services for high-throughput next-generation sequencing using the … (ChIPSEQ), RNA discovery and Multiplex sequencing … Pricing forHiSeq 2500 sequencing is based on ……..sequencing run,  …

 

MR DNA Research Laboratory Services

Services. Transcriptomics. mRNA-Seq: Stranded and non-stranded, high levels of multiplexing … ChIPSeq. Transcription factor analysis; Histone modifications

Genome Sequencing Service

Genome sequencing is described as the process of determining the order of the nucleotide bases within a certain length strand of DNA. You can sequence a short piece, the whole genome, or parts of the genome (exomes – parts of the genome that contain genes).

The knowledgeable team at MR DNA Lab (also known as Molecular Research) have been helping hundreds of researchers from around the world get the next-generation sequencing (NGS) services they need. We are one of the most reputable NGS labs providing high-quality data, and have fast turn-around without compromising quality. Our genome sequencing services are a cost-effective solution, and we try to beat anyone’s quoted pricing.

MR DNA scientists have more than 20 years of continuous experience (not combined) developing new and novel molecular methods, systematics, microsatellite screening, MHC assays, viral assays, protozoan assays and much more. Our team’s goal is to take advantage of each new technology as it arrives and leverage it to develop unique, improved and more cost effective molecular methods and tools.

Our highly-trained scientists are ready to help you with your research goals. We always try and understand the focus of your research to best provide you with the analysis you are looking for. Your results are always confidential.

At MR DNA, we offer a wide variety of genome sequencing services. Below is a general list of the different types of services we provide:

Metagenomics

ALLinONE

Whole Genome Resequencing

Exome Sequencing

Target Region Sequencing

de novo Sequencing

Whole Genome Mapping

Genotyping

Sanger Sequencing

Oligo Synthesis

Single-cell DNA Sequencing

Epigenomics

Bisulfite Sequencing

RRBS

MeDIP-Seq

Chip-Seq

Transcriptomics

RNA-Seq (Transcriptome)

RNA-Seq (Quantification)

Whole Transcriptome Sequencing

Single-cell RNA sequencing

Here is a small sample of the organisms in our database:

agrocybe chaxingu

agrocybe cylindracea

agrocybe dura

agrocybe erebia

agrocybe firma

agrocybe paludosa

agrocybe parasitica

agrocybe pediades

agrocybe praecox

agrocybe pusiola

agrocybe putaminum

agrocybe salicacicola

agrocybe smithii

agrocybe sp. cu-43

agrocybe sp. soc1251

agrocybe vervacti

Agromyces cerinus

Agromyces hippuratus

Agromyces albus

Agromyces albus

Agromyces allium

Agromyces aurantiacus

Agromyces bracchium

Agromyces cerinus

Agromyces fucosus

Agromyces fucosus

Agromyces humatus

Agromyces humatus

Agromyces italicus

Agromyces lapidis

Agromyces luteolus

Agromyces mediolanus

Agromyces neoliticus

Agromyces ramosus

Agromyces rhizospherae

Agromyces rhizospherae

Agromyces salentinus

Agromyces spp.

Agromyces subbeticus

Agromyces subbeticus

Agromyces succinolyticus

Agromyces terreus

Agromyces tropicus

Agromyces ulmi

Agromyces ulmi

Agromyces ulmi

Agropyron cristatum

Agropyron desertorum

Agropyron mongolicum

Agropyron sp.

Genome Sequencing Service

Genome sequencing is described as the process of determining the order of the nucleotide bases within a certain length strand of DNA. You can sequence a short piece, the whole genome, or parts of the genome (exomes – parts of the genome that contain genes).

The knowledgeable team at MR DNA Lab (also known as Molecular Research) have been helping hundreds of researchers from around the world get the next-generation sequencing (NGS) services they need. We are one of the most reputable NGS labs providing high-quality data, and have fast turn-around without compromising quality. Our genome sequencing services are a cost-effective solution, and we try to beat anyone’s quoted pricing.

MR DNA scientists have more than 20 years of continuous experience (not combined) developing new and novel molecular methods, systematics, microsatellite screening, MHC assays, viral assays, protozoan assays and much more. Our team’s goal is to take advantage of each new technology as it arrives and leverage it to develop unique, improved and more cost effective molecular methods and tools.

Our highly-trained scientists are ready to help you with your research goals. We always try and understand the focus of your research to best provide you with the analysis you are looking for. Your results are always confidential.

At MR DNA, we offer a wide variety of genome sequencing services. Below is a general list of the different types of services we provide:

Metagenomics

ALLinONE

Whole Genome Resequencing

Exome Sequencing

Target Region Sequencing

de novo Sequencing

Whole Genome Mapping

Genotyping

Sanger Sequencing

Oligo Synthesis

Single-cell DNA Sequencing

Epigenomics

Bisulfite Sequencing

RRBS

MeDIP-Seq

Chip-Seq

Transcriptomics

RNA-Seq (Transcriptome)

RNA-Seq (Quantification)

Whole Transcriptome Sequencing

Single-cell RNA sequencing

Here is a small sample of the organisms in our database:

Acanthamoeba sp. cvX

Acanthamoeba sp. DWDS

Acanthamoeba sp. FlaIV

Acanthamoeba sp. G.JDP

Acanthamoeba sp. J.JDP

Acanthamoeba sp. K.JDP

Acanthamoeba sp. MSA

Acanthamoeba sp. MSC

Acanthamoeba sp. Pak

Acanthamoeba sp. Rodriguez

Acanthamoeba sp. Stg

Acanthamoeba sp. strain KER

Acanthamoeba sp. strain KL

Acanthamoeba sp. strain RK

Acanthamoeba sp. TAis.JDP

Acanthamoeba sp. UPC.JDP

Acanthamoeba sp. Vazaldua

Acanthamoeba sp. Watering

Acanthamoeba sp. WSTw

Acanthamoeba spp.

Acanthamoeba stevensoni

Acanthamoeba triangularis

Acanthamoeba tubiashi

Acanthamoebidae sp.

Acanthamunnopsis longicornis

Acanthamunnopsis milleri

Acanthamunnopsis sp.

Acantharctus ornatus

Acantharctus posteli

Acantharea sp.

Acantharia sp.

Acantharian sp.

Acanthaspidia drygalskii

Acanthaspidia sp.

Acanthepeira stellata

Acanthephyra armata

Acanthephyra curtirostris

Acanthephyra eximia

Acanthephyra purpurea

Acanthina monodon

Acanthiops varius

Acanthistius ocellatus

acanthobasidium norvegicum

Acanthobasidium norvegicum

acanthobasidium phragmitis

Acanthobasidium phragmitis

Acanthobasiliola doederleini

Acanthobdella peledina

Acanthobothrium brevissime

Acanthobothrium parviuncinatum

#DNA on Twitter

Tweet-tweet! Nowadays, Twitter is not merely a social-network. It has become a must-read source of real-time industry news and information with regular updates, news and discussions from the leading minds of today. The advent of Twitter has allowed for folks to have real-time conversations with thought leaders across the field. An upcoming researcher in genomics can now engage with genomics gurus at the swipe off a finger. Online communities such as blogs have been around for a bit, but now the micro-communities like Twitter allow for real-time discussions. Get the latest insights from across the globe as they are happening. Over 35 percent of Americans per capita are active on Twitter, a close second to the UK which has almost 40 percent.

The infographic below displays the top nations actively using Twitter per capita:

Follow @MRDNA_Lab on Twitter to get the latest content surrounding applied DNA sciences, genomics news, next-generation sequencing and more.

Don’t have a Twitter account? You can sign up for free today.

Twitter is a real-time information network that connects people to the latest stories, ideas, opinions and news that they find interesting. At the heart of Twitter are small bursts of information called Tweets. Each Tweet is 140 characters long. Tweets include photos, videos and conversations that enable followers to get the whole story at a glance, and all in one place.

Twitter connects organizations to their audiences in real time—and organizations can use Twitter to quickly share information with people interested in their products and services, gather real-time market intelligence and feedback, and build relationships with customers, partners and influencers. This is done by ‘following’ members of your target market or by communicating with twitter users who are already interested and ‘following’ you. Tweets can include live links in the form of hash tags (#) and user tags or handles (@). Twitter is all about engaging with an audience in real time, with short sharp alerts and posts, providing links to further content if necessary.

Microbial Communities: Microbiome, Metagenome, Microbiota

Microbiome and microbiota explain the collective genomes of the microorganisms that inhabit an environmental niche or the microorganisms themselves. Micobiota are the microorganisms present within a particular environment. The approach to describe microbial diversity relies on analyzing the gene diversity 16S ribosomal RNA (16S rRNA) through next-generation sequencing. The “S” in 16S rRNA genes survey ( or 16S rRNA genes sequencing) represents a Svedberg unit. Microbiome refers to the entire habitat – including the microorganisms, their genes, and the surrounding environmental setting. Metagenome is the collection of genomes and genes from the members of a microbiota.

MR DNA Lab offers microbial sequencing. Microbial sequencing is the focused sequencing of a single microbe or relatively small group of microbes, in contrast with metagenomics. It can assist in the discovery of genetic variations that support the designing of antimicrobial compounds, vaccines, and even engineered microbes for industrial applications. (1) MR DNA Lab’s extensive collaborations extend all over the world, to the UK, Israel, France, Italy, Saudi Arabia, Germany and many others.

Next-Generation Sequencing Services (DNA)

Scientists are now able to elucidate the microbiome of human diseases, agricultural and other natural, environments. Especially at MR DNA Lab, scientists are dedicated to microbiome research. Their method development has opened doors to research around the world.

This initiative is one component of the MR DNA program and constitutes a major NIH effort to broaden access to rapid assay technologies. This program will fund the development and adaptation of biological assays for use in automated high throughput molecular screening (HTS). It is intended that this initiative promote the development of automated screening projects. High throughput molecular screening (HTS) is the automated, simultaneous testing of thousands of distinct molecular signatures in models of biological mechanisms. Active compounds identified through HTS can provide the starting point in the design of powerful research tools that allow pharmacological probing of basic biological mechanisms, and which can be used to establish the role of a molecular target in a disease process, or, its ability to alter the metabolism or toxicity of a therapeutic. The immense potential of HTS to impact our understanding of biological mechanisms is largely untapped because access to automated screening facilities and large compound libraries is limited in academic, government and non-profit research sectors. Many in vitro biological models are currently used to study biological pathways, the effects of genetic perturbations and to establish a disease association. These can be adapted to high throughput formats for the purpose of screening large collections of biologically active compounds. There are a number of characteristics that make an assay suitable for high throughput approaches. The assay must be robust, reproducible and have a readout that is amenable to automated analysis. In addition, it must be possible to miniaturize the assay, for example; to a 96-well plate (or higher density) format or flow-cytometric approach. Further, the assay protocol should be simple enough for automated handling. A broad range of models share many of these features, including; biochemical assays, cellular models and certain model organisms such as yeast or C. elegans. This initiative will support the development of innovative assays for use in both basic research and in therapeutics development programs, with an emphasis on novelty of assay approach and/or novel targets and mechanisms. (1)

The following list is a basic description of the sequencing services provided by Molecular Research, LP (MR DNA).

Genome Sequencing

Genome sequencing is the process through which we can elucidate the the core information (genes) of the DNA or RNA of the sample, or in the case of whole genome sequencing, the entirety of the information (genes and non-coding sequences).

Metagenomics

Metagenomics is a rapidly evolving field through which scientists can elucidate some of the previously hidden insights into the vast array of microscopic life on the planet. Every day, scientists are gaining a better understanding of ecology, evolution, diversity, and functions of the microbial universe thanks to metagenomics, which seeks to help sequence microorganisms in large groups that are often difficult to culture.

Microbial Sequencing

Microbial sequencing is the focused sequencing of a single microbe or relatively small group of microbes, in contrast with metagenomics. It can assist in the discovery of genetic variations that support the designing of antimicrobial compounds, vaccines, and even engineered microbes for industrial applications.

Genotyping

Genotyping is the technique through which the variations in an organisms DNA are determined by comparing that organisms DNA to a reference sequence. Genoptyping of an organism also reveals its alleles, the various alternative forms of genes or groups of genes. It plays a very important part in the study of diseases, and in combination with next-generation sequencing technology will help improve treatment methods.

Exome Sequencing

Selective sequencing of coding regions of the genome is an effecient and effective alternative to whole genome sequencing. Exons are the parts of coding regions which control the translation of proteins.

Transcriptome Sequencing

Transcriptome sequencing focuses on the complete array of RNA molecules, which include transfer RNA, messenger RNA, ribosomal RNA, and non-coding RNA. Transcriptome sequencing can help answer questions about gene expression, discovery of novel genes and their functions, classification of diseases, or to help identify targets for drug treatment development.

Amplicon Sequencing

Amplicon sequencing targets relatively small, specific regions of the genome usually in the hundreds of base pairs. Amplicon sequencing combined with next-generation sequencing allows for thousands of amplicons across many samples to be prepared simultaneously and indexed within hours and often within a single-run.

Bacterial/Viral Typing

Bacterial and virus typing is used in the accurate and fast identification and discrimination of strains. Enhancements in bacterial and viral typing can also assist in outbreak identification, surveillance, and in the understanding of transmission, pathogenesis, and evolutionary relationships of the target. Often specific isolates can be sequenced within a day using next-generation sequencing techniques.

De novo Sequencing

De novo is a latin expression meaning “from the beginning”. Hence, de novo sequencing is primarily focused on the sequencing of a novel genome for the first time, or genomes in which large variations are expected, such as genomes with high plasticity. It often requires specialized assembly of sequencing reads, and can be very computationally intensive, though next-generation sequencing has largely reduced the overhead associated with it.

Targeted DNA Sequencing

Targeted DNA sequencing allows the researcher to utilize the specificity of PCR in order to target the genes of their choosing. Targeted DNA sequencing provides the ability to acheive deeper sequencing coverage in order to identify those genes expressed at lower levels that may possibly have been missed by other sequencing methods.

Targeted RNA Sequencing

Targeted RNA sequencing allows the researcher to utilize the specificity of PCR in order to target the genes of their choosing. Targeted RNA sequencing provides the ability to acheive deeper sequencing coverage in order to identify those transcripts expressed at lower levels that may possibly have been missed by other sequencing methods.

Aneuploidy and CNV Analysis

Aneuploidy and Copy-umber variations (CNV) are important factors in the study of genetic disorders, disease, and phylogenetics. Next-generation sequencing has made the study and analysis of aneuploidy and CNV much easier than with previous methods.

Small RNA and miRNA Sequencing

This type of sequencing uses high-throughput methods to sequence miRNA and small RNA, which are important to tissue expression patterns, isoforms, and disease associations.

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